minion 由 The European Bioinformatics Institute（EBI）开发，可用于预测接头。

## 一、安装

linux下载网址:

http://wwwdev.ebi.ac.uk/enright-dev/kraken/reaper/binaries/reaper-13-100/linux/

## 二、用法

minion search-adapter -i SRR.fastq


criterion=sequence-density
sequence-density=52.19
sequence-density-rank=1
fanout-score=31.57
fanout-score-rank=1
prefix-density=54.75
prefix-fanout=30.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA

criterion=fanout-score
sequence-density=52.19
sequence-density-rank=1
fanout-score=31.57
fanout-score-rank=1
prefix-density=54.75
prefix-fanout=30.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA


The two criteria are unfortunately necessary due to the varying charachteristics of 3' adapter sequence in different experimental protocols. The first criterion is frequency of occurrence; the second criterion incorporates a fan-out measure that captures the typical characteristic of 3' adapter sequence of being attached to a multitude of different prefixes. When infering adapters (i.e. without using-adapter) two candidate sequences will be shown. The second will start with the linecriterion=fanout-score. The second should only be considered if the first candidate is clearly a biological sequence. This can be established by using one of theBLASTinterfaces provided for example byNCBIandENSEMBL. 也就是说，只有在第一个序列确认为是有生物学意义时，才考虑第二个序列（criterion=fanout-score）。可以用NCBI或ENSEMBL的BLAST平台来确认是否有生物学意义