【7.2】基于epitope的疫苗设计

整个思路如下:

  1. 基于各种计算,筛选出无毒性、有免疫原性、与MHCI/MHCII 高度结合、覆盖更大的人群分布、保守区域的CTL/HTL/LBL epitope。
  2. 多个epitope组合,epitope之间添加各种Linker。
  • GPGPG linkers.
  • HTL and LBL epitopes were linked by (EAAK)2.
  • Intra-LBL epitopes were spaced using (EAAK)2 or (EAAK)2E.
  • LBL and CTL epitopes were linked by AAY.
  • Intra-CTL epitopes were combined using AAY linkers
  1. 序列中添加免疫刺激的序列 CD40L和MHC I-targeting domain (MITD)
  2. 信号肽默认tPA,UTR分别为β globin 和α globin 。

参考资料

个人公众号,比较懒,很少更新,可以在上面提问题,如果回复不及时,可发邮件给我: tiehan@sina.cn