【7.2】基于epitope的疫苗设计

整个思路如下：

1. 基于各种计算，筛选出无毒性、有免疫原性、与MHCI/MHCII 高度结合、覆盖更大的人群分布、保守区域的CTL/HTL/LBL epitope。
2. 多个epitope组合，epitope之间添加各种Linker。

• GPGPG linkers.
• HTL and LBL epitopes were linked by (EAAK)2.
• Intra-LBL epitopes were spaced using (EAAK)2 or (EAAK)2E.
• LBL and CTL epitopes were linked by AAY.
• Intra-CTL epitopes were combined using AAY linkers

3. 序列中添加免疫刺激的序列 CD40L和MHC I-targeting domain (MITD)
4. 信号肽默认tPA，UTR分别为β globin 和α globin 。

参考资料

• Designing a novel mRNA vaccine against SARS-CoV-2: An immunoinformatics approach。 https://www.sciencedirect.com/science/article/pii/S0141813020336655